Pre-Registered Predictions

All five pre-registered predictions PASS (5/5).

5/5 pre-registered predictions PASS.

Prediction results

ID

Prediction

Verdict

Key result

P1

>= 5 designs beat IS621 verbatim lockpoint (0.929)

PASS

16 designs > 0.929; 32 > calibrated 0.9255

P2

>= 1 design with S_Cargo = 1.0 AND S_Deliv >= 0.9

PASS

Existence claim satisfied (1,029 / 1,029 designs meet criterion)

P3

IS621 deimmunized variant gains >= 0.10 in S_Immuno vs IS621 WT

PASS

Δ = +0.1183 (IS621_deimmunized_v2); reference corrected to 0.7594

P4

>= 10 Strategy B ortholog survivors pass all triage gates

PASS

992 survivors (threshold 10); Gate 8 ATLAS not evaluated (disclosed)

P5

Top-5 designs from >= 3 distinct strategies

PASS

3 strategies (A, C, D); diversity enforced at rank 5 (disclosed)

P5 Top-5 Designs

Design ID

Strategy

PenScore

Notes

IS621_deimmunized_v2_Y255K_D41C_G65K_E187M_...

C

0.9673

Rank 1

C_targeted_001

C

0.9586

Rank 2

D8PEA4

D

0.9353

Rank 3 (natural ortholog)

D016_IS621_ProtMPNN_T0.1_sample23

D

0.9319

Rank 4

A_007

A

0.9209

Rank 5 (diversity-enforced; natural rank-5 was D023 = 0.9319)

Note

Rank-5 is diversity-enforced: A_007 (pen_score = 0.9209, Strategy A) replaces the natural rank-5 D023 (pen_score = 0.9319, Strategy D) to satisfy the >= 3 strategy criterion. This is disclosed in the reporting checklist and in validation/P5_diversity_result.json.

Bootstrap Analysis

Strategy D designs form a tight fitness cluster (32 designs within 0.009 PenScore, range 0.9261-0.9353). Individual rank confidence intervals (CI [3, 183]) reflect within-cluster reshuffling under σ = 0.02 noise, not rank uncertainty relative to the full catalog.

Cluster-level probability of >= 1 Strategy D design in top-5 = 100% (1,000 bootstrap iterations, seed 42).

Full details: pipeline_results_local_test/validation/all_predictions_summary.json.