Triangulation Report

Step 18–20 cross-referenced the four PEN-STACK pipelines to identify mechanism discrepancies in the natural editor universe.

Summary (30 discrepancy records, 29 natural editors)

Category	Severity	Count	Description
SIZE_INCONSISTENCY	Medium	13	Atlas entry present but length_aa=None in pen-score
MECH_VS_PFAM	High	11	Atlas + high S_DSB but tier_a_gate=False (site-specific recombinases)
EVIDENCE_GAP	Low	5	IS110 confirmed, S_DSB ≥ 0.95, but cell_based=False
CARGO_INCONSISTENCY	Medium	1	intrinsic_cargo=True but S_Cargo < 0.60 (evoCAST)
AXIS_VS_TIER	High	0	No editors with tier_a_gate=False and S_DSB < 0.80 in IS110 direction

P3 outcome

P3 PASS: 30 discrepancy records across 29 natural editors (threshold: ≥ 5). Zero discrepancies in the 1,029 computational designs (by construction — designs inherit evidence flags from pen-assemble catalog).